Yup, then took it off and put a fixed cog on
As long as you get the chain line and chain length right then you shouldn't have any problems.
Standard english hub thread is 1.37" X 24 TPI and the same is true for multiple freewheels and single speed sprockets.
I've used a set of miche hubs from the mid 80's and a brand new dicta single speed freewheel with no problems whatsoever.
In the garage:
2011 Genesis flyer fixed wheel; 2012 Wilier Gran Turismo (shiny
Been and gone:
Ribble columbus slx; 1982 Viscount KP crisps replica team bike; 2009 Marin Wolf Ridge 6.9; Veneto Columbus altec 2 road bike; 1993 Specialized stumpjumper; 1980s Raleigh team replica